Greater than 2:1 in the presence of phosphatidyl glycerolĬhromosome analysis (indicative of inherited genetic mutations) Less than 0.03 ΔOD at term (approximately 37–40 weeks, using the Liley Chart when gestational age is equal to or greater than 27 weeks) Less than 0.06 ΔOD in late pregnancy (approximately 28–36 weeks, using the Liley Chart when gestational age is equal to or greater than 27 weeks) ![]() Less than 0.05 ΔOD in early to mid-pregnancy (approximately 14–27 weeks, using the Queenan curve when gestational age is less than 27 weeks) Method: Macroscopic observation of fluid for color and appearance, immunochemiluminometric assay (ICMA) for alpha 1-fetoprotein, electrophoresis for acetylcholinesterase, chromatography for lecithin/sphingomyelin (L/S) ratio and phosphatidylglycerol, cell culture for chromosome analysis, dipstick for leukocyte esterase, and automated cell counter for WBC count and lamellar bodies.Īcetylcholinesterase (in conjunction with AFP indicative of possible neural tube defect) There are no food, fluid, activity, or medication restrictions unless by medical direction. ![]() To assess for preterm infant fetal lung maturity (FLM) to assist in evaluating for potential diagnosis of respiratory distress syndrome (RDS). ![]() To assist in identification of fetal gender, genetic disorders such as hemophilia and sickle cell anemia, chromosomal disorders such as Down syndrome, anatomical abnormalities such as spina bifida, alloimmune hemolytic disease of the newborn (HDN), and hereditary metabolic disorders such as cystic fibrosis.
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